Protocol based on Johansson et al., 2015. Materials
Plastic 50 ml centrifuge tubes Plastic 15ml falcon tubes 5ml pipette tips Distilled water Plastic bacterial inoculation loops Petri dishes Corning Costar Flat Bottom 6-well Cell Culture Plates (Fisher) Tea strainer Conductivity meter: Jenway 3540 pH and conductivity meter Conductivity probe: Glass bodied 4mm diameter, 120mm reach probe (Part code 027 815) 10μS and 84μS conductivity standard (Part code 025 139 and 025 164 respectively)
Experimental design
Always take along a positive and a negative control Make sure the plants used look good.
Protocol Protocol based on Smith and Heese, 2014 and Albert & Fürst, 2017 Materials
Distilled water Plastic bacterial inoculation loops Scalpel Petri dishes 96 well plates (Corning 96-Well Solid White Polystyrene Microplates) Luminometer (TECAN infinite M200) 500x L-012 stock solution (10μM): 3.1mg L-012 (a new luminol derivative with a higher activity which reacts with various types of reactive oxygen species) was completely dissolved in 1 ml of DMSO and used at a final concentration of 20μM.